BSc (University of Winnipeg) 1976
MSc. (University of Manitoba) 1979
Ph.D (University of Manitoba) 1984
|Teaching & Research
Molecular/Cell Biology, Immunology: Cell secretion, diabetes, immunocontraceptives, NK cells
here are three major areas of research
in my laboratory.
Interestingly, we have detected low levels of the CASP binding partner, Cytohesin-1, in a complex with immunoprecipitated SNX27. The CASP–SNX27 interaction could support a mechanism where members of the Cytohesin/ARNO family are dynamically recruited to early endosomal membranes for vesicular trafficking in lymphocytes. .
A CASP-aided interaction between Cytohesin/ARNO family members and SNX27 could bring both PX and PH domains into one complex and may contribute to the rapid transition of budding vesicles to early endosomes, as shown in our proposed model (see figure) that was recently published.
(2) Tilapia Islet Cell Biology:
(Funded by JDF USA, Collaboration with Dr. Jim Wright, Pathology, University of Calgary). Dr. Wright has successfully used transplantation of tilapia pancreatic islets to treat diabetic animals (xenograft transplantation). The major advantages are that islets in some fish occur as a discreet organ (Brockman Bodies) that can be easily extracted and contain primarily islet cells (unlike human pancreases). To use this therapy successfully in humans, it would be extremely beneficial if the insulin produced was human. My group cloned the complete tilapia insulin gene and by using site-directed mutagenesis and linker substitutions we have completed the "humanization" of this tilapia insulin gene. We have re-introduced this new hybrid gene into tilapia (transgenic) and have produced homozygous animals that secrete human insulin. We are presently studying the regulation of blood glucose levels in tilapia.
(3) Vaccine Development (Funded by AIF. Collaboration with ImmunoVaccine Technologies IVT- Halifax, NS). Several years ago we developed at Dalhousie a single immunization birth control vaccine for seals. This vaccine was able to elicit a strong response that lasted for more than 10 years. Using this technology we have developed potent vaccines for several animal diseases. We are presently developing vaccines that can be used to treat cancer in humans.
|Examples of Students' Research
Honors BSc Students
If you are interested in any of the above research please contact Dr. Pohajdak by email.
MacNeil AJ, and Pohajdak B. 2009. Getting a GRASP on CASP: properties and role of the cytohesin-associated scaffolding protein in immunity. Immunology and Cell Biol. 87: 72-80
Olga Hrytsenko, Bill Pohajdak, Bao-You Xu, Carol Morrison, Brenna vanTol and James R. Wright Jr.. 2009. Cloning and Molecular Characterization of the Glucose Transporter 1 in Tilapia (Oreochromis niloticus). General and Comparative Endocrinology (in press).
Olga Hrytsenko, Bill Pohajdak and James R. Wright Jr. 2009. Production of transgenic tilapia homozygous for a humanized insulin gene. Transgenic Res. (in press).
Olga Hrytsenko; James R Wright, Bill Pohajdak 2008 Regulation of insulin gene expression and insulin production in Nile tilapia (Oreochromis niloticus) General and Comparative Endocrinology. 155(2):328-40.
James R. Wright Jr, Jaime Snowdon, Olga Hrytsenko, Carol M. Morrison & Bill Pohajdak 2008 Immunohistochemical staining for tilapia and human insulin demonstrates that a tilapia transgenic for humanized insulin is a mosaic. Transgenic Res. 2008 Oct;17(5):991-992.
Adam J. MacNeil, Lori A. McEachern, and Bill Pohajdak 2008. Gene duplication in early vertebrates results in tissue-specific subfunctionalized adaptor proteins: CASP and GRASP. J Mol Evol. 2008 Aug; 67(2):168-78
Adam J. MacNeil, Bill Pohajdak 2007. Polarization of endosomal SNX27 in migrating and tumor-engaged Natural Killer cells. Biochemical and Biophysical Research Communications 361(1):146-50.
Hrytsenko O, Wright JR Jr, Morrison CM, Pohajdak B. Insulin expression in the brain and pituitary cells of tilapia (Oreochromis niloticus). Brain Res. 2007 11(1):31-40.
Mansour M, Pohajdak B, Kast WM, Fuentes-Ortega A, Korets-Smith E,Weir GM, . 2007 Therapy of established B16-F10 melanoma tumors by a single vaccination of CTL/T helper peptides in VacciMax(R). J Transl Med Apr 23;5(1):20
Adam MacNeil, Marc Mansour and Bill Pohajdak. 2007 Sorting Nexin 27 interacts with the Cytohesin Associated Scaffolding Protein (CASP) in lymphocytes. Biochemical and Biophysical Research Communications. 359(4):848-853.
Pirouz Daftarian, Marc Mansour, Anita C. Benoit, Bill Pohajdak, David W. Hoskin, Robert G. Brown and W. Martin Kast. 2006. Eradication of established HPV 16-expressing tumors by a single administration of a vaccine composed of a liposome-encapsulated CTL-T helper fusion peptide in a water-in-oil emulsion. Vaccine 12;24(24):5235-44.