PROFESSOR AND CHAIR
Cross-appointment Department of Pharmacology
BSc (Mt. Allison, 1970), MSc (Windsor, 1973)
PhD (Windsor, 1976)
|Teaching & Research
cell/molecular biology, cell stress, diapause, embryo development, Artemia, molecular chaperones, small heat shock proteins, HSP70, LEA proteins, breast cancer.
esearch in my laboratory centers on the molecular analysis
of diapause, a physiological state characterized by dormancy (suspended
animation) and enhanced stress tolerance. Gene expression during initiation, maintenance
and termination of diapause is investigated in embryos of the crustacean Artemia franciscana, as are the functions of molecular chaperones
and LEA proteins during diapause. Stemming from these studies is the molecular
analyses of small heat shock protein and HSP70 structure and function. Artemin, a novel ferritin homologue
synthesized in diapausing Artemia embryos, and which exhibits chaperone
activity, is examined by similar methodologies. We have recently adapted RNA interference (RNAi) methodologies to study the function of molecular chaperones and LEA proteins in vivo.
|Examples of Students' Research
Some Graduates' Occupations
King, A.M. and MacRae, T.H. 2012. The Small Heat Shock Protein p26 Aids Development of Encysting Artemia Embryos, Prevents Spontaneous Diapause Termination and Protects Against Stress. PLoS ONE 7(8): e43723.
Baruah, K., Ranjan, J., Sorgeloos, P., MacRae, T.H. and Bossier, P. 2011. Priming the Prophenoloxidase System of Artemia franciscana by Heat Shock Proteins Protects Against Vibrio campbellii Challenge. Fish Shellfish Immunol. 31: 134-141.
Sung, Y.Y. and MacRae, T.H. 2011. Heat Shock Proteins and Disease Control in Aquatic Organisms. Aquaculture Res. Develop. http://dx.doi.org/10.4172/2155-9546.S2-006. Published on line, 10 pages. (Review).
Hu, Y., Bojikova-Fournier, S., King, A.M. and MacRae, T.H. 2011. The Structural Stability and Chaperone Activity of Artemin, a Ferritin Homologue from Diapause-Destined Artemia Embryos, Depend on Different Cysteine Residues. Cell Stress Chaperones. 16: 133-141.
Baruah, K., Ranjan, J., Sorgeloos, P., MacRae, T.H.and Bossier, P. 2011. Priming the Prophenoloxidase System of Artemia franciscana by Heat Shock Proteins Against Vibrio campbellii Challenge. Fish Shellfish Immunol. 31: 134-141.
Sung. Y.Y., MacRae, T.H., Sorgeloos, P. and Bossier, P. 2011. Stress Response for Disease Control in Aquaculture. Rev. Aquaculture 3: 120-137. (Review).
Wu, Y. and MacRae, T.H. 2010. Truncation Attenuates Molecular Chaperoning and Apoptosis Inhibition by p26, a Small Heat Shock Protein from Artemia franciscana. Biochem. Cell Biol. 88: 937-946.
Warner, A.H., Miroshnychenko, O., Kozarova, A., Vacratsis, P.O., MacRae, T.H., Kim, J. and Clegg, J.S. 2010. Evidence for Multiple Group 1 Late Embryogenesis Abundant Proteins in Encysted Embryos of Artemia and Their Organelles. J. Biochem. 148: 581-592.
Robbins, H., Van Stappen, G ., Sorgeloos, P., Sung, Y.Y., MacRae, T.H. and Bossier, P. 2010. Diapause terminaton and development of encysted Artemia embryos: Roles for nitric oxide and hydrogen peroxide. J. Exp. Biol. 213: 1464-1470.
Petrovick, M.S., Nargi, F.E., Towle, T., Hogan, K., Bohane, M., Wright, D.J., MacRae, T.H., Potts, M. and Helm, R.F. 2010. Improving the long-term storage of a mammalian biosensor cell line via genetic engineering. Biotech. Bioeng. 106: 474-481.
MacRae, T.H. 2010. Gene expression, metabolic regulation and stress tolerance during diapause. Cell. Mol. Life Sci. 67: 2405-2424. (Review).
Garant, K.A. and MacRae, T.H. 2009. Cloning and sequencing of tubulin cDNAs from Artemia franciscana: Evidence for differential expression of α- and ß-tubulin genes. Biochem. Cell Biol. 87: 989-997.
Sung, Y.Y., Dhaene, T., Defoirdt, T., Boon, N., MacRae, T.H., Sorgeloos, P. and Bossier, P. 2009. Ingestion of bacteria over-producing Dnak attenuates Vibrio infection of Artemia franciscana larvae. Cell Stress & Chaperones 14: 603-609. (Cover).
Sung, Y.Y., Ashame, M.F., Chen, S., MacRae, T.H., Sorgeloos, P. and Bossier, P. 2009. Feeding Artemia franciscana (Kellogg) larvae with bacterial heat shock protein protects from Vibrio campbellii (Baumann) infection. J. Fish Dis. 32: 675-685.
Brun, N.T., Bricelj, V.M., MacRae, T.H. and Ross, N.W. 2009. Acquisition of thermotolerance in bay scallops, Argopecten irradians, via differential induction of heat shock proteins. J. Exp. Mar. Biol. Ecol. 371: 77-83.
Qiu, Z. and MacRae, T.H. 2008. ArHsp22, a developmentally regulated small heat shock protein produced in diapause-destined Artemia embryos, is stress inducible in adults. FEBS J. 275: 3556-3566.
Brun, N.T., Bricelj, V.M., MacRae, T.H. and Ross, N.W. 2008. Heat shock protein responses in thermally stressed bay scallops, Argopecten irradians, and sea scallops, Placopecten magellanicus. J. Exp. Mar. Biol. Ecol. 358: 151-162.
Qiu, Z. and MacRae, T.H. 2008. ArHsp21, a developmentally regulated small heat shock protein synthesized in developing embryos of Artemia franciscana. Biochem. J. 411: 605-611.
Sung, Y.Y., Pineda, C., MacRae, T.H., Sorgeloos, P. and Bossier, P. 2008. Exposure of gnotobiotic Artemia franciscana larvae to abiotic stress promotes heat shock protein 70 synthesis and enhances resistance to pathogenic Vibrio campbellii. Cell Stress & Chaperones 13: 59-66.
Qiu, Z., Tsoi, S.C.M. and MacRae, T.H. 2007. Gene expression in diapause-destined embryos of the crustacean, Artemia franciscana. Mech. Develop. 124: 856-867.
Qiu, Z. and MacRae, T.H. 2007. Developmentally regulated synthesis
of p8, a stress-associated transcription cofactor in diapause-destined
embryos of Artemia franciscana. Cell Stress & Chaperones 12: 255-264.
Qiu, Z., Bossier, P., Wang, X., Bojikova-Fournier, S. and MacRae, T. H. 2006. Diversity, structure and expression of the gene for p26, a small heat shock protein from Artemia. Genomics 88: 230-240.
Sun, Y., Bojikova-Fournier, S. and MacRae, T.H. 2006. Structural and functional roles for ß-strand 7 in the α-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana. FEBS J. 273: 1020-1034.
Villeneuve, T.S., Ma, X., Sun, Y., Oulton, M.M., Oliver, A.E. and MacRae, T.H. 2006. Inhibition of apoptosis by p26: implications for small heat shock protein function during Artemia development. Cell Stress & Chaperones 11:72-80. (Cover).
Ma, X., Jamil, K., MacRae, T.H., Clegg, J.S., Russell, J.M., Villeneuve, T.S., Euloth, M., Sun, Y., Crowe, J.H., Tablin, F. and Oliver, A.E. 2005. A small stress protein acts synergistically with trehalose to confer desiccation tolerance on mammalian cells. Cryobiology 51: 15-28.
Sun, Y. and MacRae, T. H. 2005. Small heat shock proteins: molecular structure and chaperone function. Cell. Mol. Life Sci. 62: 2460-2476. (Review)
MacRae, T. H. 2005. Diapause: diverse states of developmental and metabolic arrest. J. Biol. Res. 3: 3-14. (Review; cover).
Sun, Y. and MacRae, T. H. 2005. The small heat shock proteins and their role in human disease. FEBS J. 272: 2613-2627. (Review).